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[2012/07/25] A Breakthrough in Cultivation of Mesenchymal Stem Cells for Clinical Uses

[2012/07/25]

A Breakthrough in Cultivation of Mesenchymal Stem Cells for Clinical Uses

Professor Shih-Chieh Hung and his team at Taipei Veterans General Hospital and National Yang Ming University on Monday (July 16, 2012) announced a breakthrough in cultivation of stem cells, which will provide a better source of such cells for disease treatments.

Dr. Hung and Dr. Chih-Chien Tsai, a postdoctoral fellow at National Yang-Ming University, who joined Dr. Hung's team 5 years ago, published their research in Molecular Cell journal. They found that mesenchymal stem cells (MSCs) at early passage (E), in hypoxic culture (H) and with p21 knockdown (p21KD) increased in Oct4 and Nanog expression along with increased proliferation and differentiation potential but decreased spontaneous differentiation compared to MSCs at late passage (L), in normoxic culture (N) and scrambled shRNA-overexpressed (Scr) MSCs. Knockdown of Oct4 and Nanog in E, H and p21KD MSCs decreased proliferation and differentiation potential, and enhanced spontaneous differentiation, whereas overexpression of Oct4 and Nanog in L, N and Scr MSCs increased proliferation and differentiation potential, and suppressed spontaneous differentiation. Oct4 and Nanog upregulate Dnmt1 through direct binding to its promoter, thereby leading to the repressed expression of p16 and p21, and genes associated with development and lineage differentiation.

Professor Hung also published their researches in many famous journals in the past five years, such as Blood, Stem Cells, Aging Cell, Cell transplantation and PLoS ONE. These researches indicated that hypoxic cultures of MSCs could maintain stem cell properties, such as self-renewal and differentiation potential, inhibit replicative senescence, and enhance paracrine effects on angiogenesis, would and fracture healing. MSCs cultured under hypoxic conditions also increased in cell migration and engraftment after cell transplantation. These data have also been submitted for patent application both in Taiwan and United States.

These data from Dr. Hung's team demonstrate the benefits of hypoxic culture on MSCs. The hypoxic cultivation will help developing new techniques in expansion or production of high quality MSCs for further clinical uses for a lot of diseases in Taiwan and the world.

 

 

Professor Hung(left) and his postdoctoral fellow Tsai Chih-Chien

Professor Hung(left) and his postdoctoral fellow Tsai Chih-Chien


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